WebbOligoPerfect Thermo Fisher Scientific - US OligoPerfect Primer Designer Design the perfect primers for your PCR, CE Sequencing and Cloning (Restriction Enzyme Cloning, … WebbExample oligo design: Note that the NGG PAM is not included in the designed oligos. Oligonucleotide ordering tips: Standard de-salted oligos (usually the most inexpensive synthesis) are sufficient for cloning. If not already resuspended, dilute each oligo to 100 μM in sterile water or TE. Golden-Gate sgRNA cloning protocol 1. Oligo anneal

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WebbOur oligos are made to your specifications, with rigorous quality control, and quick turnaround for use in a variety of applications, including PCR, cloning, sequencing, and … WebbThe α-crystallin domain-containing (ACD-containing) gene family, which includes typical small heat shock proteins (sHSPs), is the most ubiquitous and diverse family of putative chaperones in all organisms, including eukaryotes and prokaryotes. In the present study, approximately 54-117 ACD-containing genes were identified in five penaeid ... steve and kathy doocy

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WebbConfigure custom research-grade oligos for any application, including unlabeled PCR primers, highly modified oligos, or DNA/RNA hybrid sequences, with our extensive selection of nucleic acid derivatives and specialty oligo building blocks. Key benefits: ISO 9001:2015-certified quality management system 1000+ oligo modifications WebbThe presence of the primary amino group allows the user to label the oligo with a variety of different ligands for affinity, reporter or protein moieties (as NHS esters or isothiocyanates), depending on the application. WebbPrimer design tips. In general, a length of 18–30 nucleotides for primers is good. Try to make the melting temperature (T m) of the primers between 65°C and 75°C, and within … steve and kate\u0027s camp atlanta