WebOct 21, 2016 · Using this phosphomolybdic acid-picrosirius red technique, collagenous septa as thin as 0.2-0.5 micron and fine collagen fibers making up the septa were clearly … WebPhosphomolybdic acid solution is a ready-to-use spray for the visualization of steroids, alkaloids, antioxidants, and terpenes resolved by thin-layer chromatography (TLC) and high-performance TLC (HPTLC). Safety Information Pictograms GHS02,GHS03,GHS05,GHS07 Signal Word Danger Hazard Statements H225 - H272 - H314 - H336 Precautionary …
TLC Stains - University of Washington
WebJan 2, 2024 · The recipe for this stain is really easy: Weigh 10-15 g of vanillin, dissolve it 250 mL of ethanol, and add 2.5 mL of concentrated sulfuric acid. Stir and you are good to go! To use it just dip your eluted TLC plate, and heat up with a heating gun. Phosphomolybdic Acid (PMA) This is another great general purpose stain. WebPhosphomolybdic acid solution is primarily used in spectrophotometric determination of phosphate ions. PMA is also component in the 3-color histology stain called Masson's trichrome; employed in the differentiation step, Phosphomolybdic acid is one of the solutions fixed tissues are immersed in prior to subsequent staining. highlander 2011 price
Optimization of TLC Detection by Phosphomolybdic Acid …
Webnuclear staining. In this study, we evaluated the ability of phosphomolybdic acid (PMA) pretreatment to prevent nonspecific nuclear staining by PSR. In a manual evaluation of 27 … Phosphomolybdic is used as a stain for developing thin-layer chromatography plates, staining phenolics, hydrocarbon waxes, alkaloids, and steroids. Conjugated unsaturated compounds reduce PMA to molybdenum blue. The color intensifies with increasing number of double bonds in the molecule being stained. Phosphomolybdic acid is also occasionally used in acid-catalyzed reactions in organic synthesis… WebPhosphomolybdic acid solution is a versatile staining reagent used in chromatography and other applications available in typical concentrations of 10-20% in polar organic solvents such as ethanol. ... Dodecamolybdophosphoric acid, Dodeca molybdophosphoric acid, PMA, Molybdo(VI)phosphoric acid, Phosphomolybdic acid 20 wt. % in ethanol ... highlander 2012 price