Organotypic raft cultures是什么

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August undefined, 2024

WitrynaThe productive phase of human papillomavirus (HPV) infection is dependent on squamous differentiation of epithelial keratinocytes. Organotypic culture systems of primary human keratinocytes (PHKs) or immortalized keratinocytes that contain HPV genomes were developed to recapitulate this permissive environment. WitrynaDownload scientific diagram Adenovirus infection of lacerated raft cultures at a high MOI. (A) Time course of infection. Fully differentiated raft cultures were lacerated with a sterile scalpel ...

of Organotypic Raft Culture - Springer

Witryna1 sie 2014 · Using organotypic (raft) epithelial tissue cultures for the biosynthesis and isolation of infectious human papillomaviruses Curr Protoc Microbiol. 2014 Aug 1; ... showup hack

Organotypic raft culture of PHKs — The Open Lab Book v1.0

WitrynaOrganotypic raft cultures show epithelium-specific, harmful effects of topical drugs, provided the compounds are supplied onto the culture surface and not into the … Witrynaorganotypic culture的中文翻譯，organotypic culture是什麼意思，怎麽用漢語翻譯organotypic culture，organotypic culture的中文意思，organotypic culture的中 … Witryna21 lut 2024 · Here, we generated organotypic epithelial raft cultures (OERCs) of MCC by using primary human keratinocytes and both virus-positive and virus-negative … showup entertainment

organotypic culture中文, organotypic culture中文意思

Organotypic epithelial raft cultures as a model for evaluating

Witryna1 lis 2005 · The antiviral effects of the different compounds in the organotypic raft cultures were quantified by measuring viral titers by plaque assay for HSV and viral … Witryna5 wrz 2024 · For epithelial cancers, organotypic raft cultures can constitute a suitable in vitro model. Tumor-organoid cultures are easiest to handle, and can mimic stem-cell properties and virus–ECM interactions. Of course, further investigations have to be done to establish three-dimensional cell cultures as a common alternative to more … showup01Witryna1 sty 2024 · The protocols described below give rise to 3D esophageal epithelial cultures that can be grown as organoids or organotypic raft cultures, and these have been … showupdatedialog

"Witryna1 lis 2003 · There is a growing body of evidence showing that optical spectroscopy has the potential to be a useful in vivo diagnostic tool. Yet, so far there is no definitive cellular and biochemical understanding for the differences seen in the spectra from different tissue categories and disease states. In this study, we examine the use of … " - Organotypic raft cultures是什么

Organotypic raft cultures是什么

WitrynaWe have developed an in vitro organotypic raft culture system that allows researchers to study HPV in its natural environment. Not only does this system reproduce the differentiation-dependent replication cycle of HPV, but it also allows for the production of high titers of native HPV virions. Currently, much of the HPV research has been done ... Witryna22 lut 2012 · For instance, the ability to grow three-dimensional organotypic raft cultures of keratinocytes has been an important milestone in the study of human …

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Witryna21 lut 2024 · Organotypic epithelial raft cultures (OERCs) are 3D in vitro culture systems that permit terminal differentiation of keratinocytes—the major cell type of the epidermis—when seeded on top of collagen beds at the air–liquid interface. … WitrynaTY - JOUR. T1 - Organotypic (raft) culture of biopsy-derived upper respiratory epithelium. AU - Schweinfurth, John Mark. AU - Meyers, Craig. PY - 2006/9/1

WitrynaThe organotypic (raft) culture system has been used to develop an in vitro system that is capable of reproducing the entire human papillomavirus (HPV) life cycle, including virion morphogenesis. This system utilizes HPV-containing cell lines that are either derived from biopsies or created by the transfection of keratinocytes with HPV … Witryna1 lis 2024 · Primary human foreskin keratinocytes containing HPV18 episomes were grown in a three-dimensional organotypic raft culture to support full epithelial differentiation and the complete HPV18 life cycle. Raft cultures were fixed and paraffin embedded prior to sectioning. Cellular nuclei are stained in blue (DAPI), HPV18 …

WitrynaWe have developed an in vitro organotypic (raft) culture system capable of reproducing the differentiation-dependent replication cycle of human papillomavirus. A dermal … WitrynaA study from 2008 initially described the methodology of this culture system adapted for prions, termed POSCA (Prion Organotypic Slice Culture Assay), and the feasibility of its use in studying ...

Witryna8 kwi 2024 · 1. Introduction. Since the late 1970's, there has been an evolution of growing human keratinocytes in a three-dimensional (3D) rather than two-dimensional manner for ‘the reconstitution of living skin’ [] in a cell culture dish with nomenclatures such as ‘organotypic culture’ [], ‘keratinocyte raft cultures’ [], ‘organotypic raft cultures’ [] …

WitrynaOrganotypic raft cultures resembling normal and dysplastic epithelial cervical tissue were constructed and grown at an air-liquid interface for 2 weeks. Raman spectra of … showupcodeWitrynaOrganotypic epithelial raft cultures accurately reproduce the process of epithelial differentiation in vitro and can be prepared from normal keratinocytes, explanted … showuploadlistWitryna1 sie 2014 · Using Organotypic (Raft) Epithelial Tissue Cultures for the Biosynthesis and Isolation of Infectious Human Papillomaviruses. Michelle A. Ozbun, ... The apparent floating nature of the cell-matrix in this method led to the nickname “raft” cultures. The general technique can be applied to normal low passage keratinocytes, … showup messeWitryna22 lut 2012 · The life cycle of HPV is tightly linked to the differentiation of squamous epithelium(2). Organotypic epithelial raft cultures as demonstrated here reproduce … showup llcWitryna21 lut 2024 · Organotypic epithelial raft cultures (OERCs) of Merkel cell carcinoma (MCC) cell lines co-cultured with primary human keratinocytes (PHKs) at ratios of 1:1, … showup legalWitrynaThe organotypic (raft) culture system has been used to develop an in vitro system that is capable of reproducing the entire human papillomavirus (HPV) life cycle, including … showupmotion gmbhWitrynaOrganotypic Cultures Organotypic cultures of HPV-transformed and normal kera-tinocytes were prepared as described.15 Briefly, dermal equivalents were produced using collagen I (ICN, Asse-Releigem, Belgium) containing 4 104 normal human fi-broblasts. Then, 2.5 105 to 1 106 keratinocytes (HPV-transformed or normal) were seeded on … showup nighter anthem/kazsin